Emerging research suggests a compelling link between the ketogenic diet and alterations in DNA methylation patterns. This fascinating dietary approach, characterized by high fat intake and restricted carbohydrate consumption, may profoundly influence gene expression by modifying the epigenome. While the precise mechanisms remain under investigation
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Treatment with sodium bisulfite deaminates unmethylated cytosine to uracil, Whilst 5mC is not really impacted. Subsequent PCR amplification will substitute the uracils by thymines. Reads from NGS libraries produced in parallel with and without bisulfite therapy are mapped on to reference genomes and also the contrasting C/T positions in between the