DETAILS, FICTION AND GENETIC METHYLATION TEST

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Treatment with sodium bisulfite deaminates unmethylated cytosine to uracil, Whilst 5mC is not really impacted. Subsequent PCR amplification will substitute the uracils by thymines. Reads from NGS libraries produced in parallel with and without bisulfite therapy are mapped on to reference genomes and also the contrasting C/T positions in between the